Transcription-mediated replication hindrance: a major driver of genome instability

Genome replication involves dealing with obstacles that can result from DNA damage but also from chromatin alterations, topological stress, tightly bound proteins or non-B DNA structures such as R loops. Experimental evidence reveals that an engaged transcription machinery at the DNA can either enhance such obstacles or be an obstacle itself. Thus, transcription can become a potentially hazardous process promoting localized replication fork hindrance and stress, which would ultimately cause genome instability, a hallmark of cancer cells. Understanding the causes behind transcription–replication conflicts as well as how the cell resolves them to sustain genome integrity is the aim of this review

Trade and Private R&D in Mexico

Using the National Survey on Employment, Wages, Technology and Training (Enestyc), this paper tries to find the relationship between increasing trade and the proportion of total income Mexican manufacturing firms invest on R&D. Based on two cross-sectional and a panel estimation procedures, the results confirm the idea that increasing the exposure to foreign markets affect the innovative efforts of Mexican firms. We also find that the firms engaging in some kind of R&D do not conform a random sample. More specifically, our results show that, in 1992, the probability of finding a firm engaging resources in some kind of R&D increased with size, a market diversification measure, and a measure of industrial market power at a 2-digit level, while the intensity of the R&D effort depended, on market power and an industry concentration measure. For the 1999 estimation our results show that the probability of R&D investment at a firm level increased with size, a market diversification measure, and exposure to foreign competition, while the magnitude of the R&D effort of a firm was determined by the decrease in average import tariffs at the industry level and by the exporting efforts of the firm. We find strong complementarities between public and private innovation efforts in both years, but find that younger firms are doing stronger R&D efforts in 1999. The 1992- 99 balanced panel results show that exporting firms invest more in R&D while import competing firms invest less, once size, market power and other control variables are taken into account. Our estimation indicates that exporting give firms a great incentive to innovate, and that not only large, but also small firms contribute to the R&D efforts of a nation.R&D, trade liberalization, foreign direct investment, exposure to foreing markets

Rpd3L and Hda1 histone deacetylases facilitate repair of broken forks by promoting sister chromatid cohesion

Genome stability involves accurate replication and DNA repair. Broken replication forks, such as those encountering a nick, lead to double strand breaks (DSBs), which are preferentially repaired by sister-chromatid recombination (SCR). To decipher the role of chromatin in eukaryotic DSB repair, here we analyze a collection of yeast chromatin-modifying mutants using a previously developed system for the molecular analysis of repair of replication-born DSBs by SCR based on a mini-HO site. We confirm the candidates through FLP-based systems based on a mutated version of the FLP flipase that causes nicks on either the leading or lagging DNA strands. We demonstrate that Rpd3L and Hda1 histone deacetylase (HDAC) complexes contribute to the repair of replication-born DSBs by facilitating cohesin loading, with no effect on other types of homology-dependent repair, thus preventing genome instability. We conclude that histone deacetylation favors general sister chromatid cohesion as a necessary step in SCR

"L'Umorismo" de Pirandello, una poética de tradición cervantina

El ensayo de Pirandello "L'umorismo" es muy importante para la literatura italiana del principio de este siglo, porque su poesía se explica claramente allí. La idea de humorismo es bastante diferente de la ironía y la comicidad, es un profundo pensamiento y produce un sentimiento contradictorio que el escritor utiliza para hacer su propia crítica de ficción. Este punto de vista fue una novedad en ese momento, sin embargo, Pirandello basa su arte en una gran tradición donde Cervantes se revela como un maestro.Pirandello's essay “L'Umorismo” is very important for the Italian Literature of the beginning of this century because his poetry is clearly explained there. The idea of humorism is rather different from irony and comicality, it is a deeply thought and produces a contradictory feeling that writer uses to do his own fictional criticism. This view was a novelty at that time, nevertheless, Pirandello bases his art on a large tradition where Cervantes is signed as a master.notPeerReviewe

Invisible pollution : emerging marine pollutants

Since the start of the Anthropocene, the planet has been threatened by a number of risks associated with human activity. Among them, chemical pollution is posing conceptual and technical challenges that are particularly difficult to characterise on a global scale. We must ask ourselves, what is a pollutant? What properties are relevant in their definition? Studies on the abundance, persistence, mobility in the environment, and bioaccumulation potential of the chemical compounds we use every day are changing the paradigm of what we consider pollutants. Thus, compounds that do not cause acute toxicity can still be dangerous for the ecosystem when they continually reach the environment, exist in very high concentrations, or disperse easily. Thus, raising awareness about the forgotten pollution that we unknowingly generate but which is affecting our oceans will be essential to protect the planet

Tourism Climatology: Past, Present and Future

Founded in 1999 at the 15th International Congress of Biometeorology, the Commission on Climate, Tourism and Recreation (CCTR) is an international working group that seeks to advance the current state of knowledge in the field of tourism/recreation climatology. Beginning in the 1970s, climatologists explored how climate impacts a myriad of economic sectors, including tourism, with research predominantly centered on how climatological information could be used within the context of tourism planning processes (Lamb 2002). After a brief decline in research activity in the 1980s, a new phase of research growth, propelled by climate change, emerged in the 1990s (Scott & Lemieux 2010). As noted by de Freitas (2017), the field is now truly multidisciplinary, with a range of disciplines contributing diverse methodologies to understand the climate and tourism nexus. Over the past five decades, the two-way relationship between climate and tourism that was once assumed is now empirically observed, objectively tested, and incorporates concepts and theoretical frameworks that support continued model development and global comparisons. The CCTR has facilitated and engaged the growing number of researchers in the field, building collaborative partnerships with industry leaders, identifying key knowledge gaps, and setting research priorities to continually advance the state of knowledg

Efficiently Storing Well-Composed Polyhedral Complexes Computed Over 3D Binary Images

A 3D binary image I can be naturally represented by a combinatorial-algebraic structure called cubical complex and denoted by Q(I ), whose basic building blocks are vertices, edges, square faces and cubes. In Gonzalez-Diaz et al. (Discret Appl Math 183:59–77, 2015), we presented a method to “locally repair” Q(I ) to obtain a polyhedral complex P(I ) (whose basic building blocks are vertices, edges, specific polygons and polyhedra), homotopy equivalent to Q(I ), satisfying that its boundary surface is a 2D manifold. P(I ) is called a well-composed polyhedral complex over the picture I . Besides, we developed a new codification system for P(I ), encoding geometric information of the cells of P(I ) under the form of a 3D grayscale image, and the boundary face relations of the cells of P(I ) under the form of a set of structuring elements. In this paper, we build upon (Gonzalez-Diaz et al. 2015) and prove that, to retrieve topological and geometric information of P(I ), it is enough to store just one 3D point per polyhedron and hence neither grayscale image nor set of structuring elements are needed. From this “minimal” codification of P(I ), we finally present a method to compute the 2-cells in the boundary surface of P(I ).Ministerio de Economía y Competitividad MTM2015-67072-

Encoding Specific 3D Polyhedral Complexes Using 3D Binary Images

We build upon the work developed in [4] in which we presented a method to “locally repair” the cubical complex Q(I) associated to a 3D binary image I, to obtain a “well-composed” polyhedral complex P(I), homotopy equivalent to Q(I). There, we developed a new codification system for P(I), called ExtendedCubeMap (ECM) representation, that encodes: (1) the (geometric) information of the cells of P(I) (i.e., which cells are presented and where), under the form of a 3D grayscale image gP ; (2) the boundary face relations between the cells of P(I), under the form of a set BP of structuring elements. In this paper, we simplify ECM representations, proving that geometric and topological information of cells can be encoded using just a 3D binary image, without the need of using colors or sets of structuring elements. We also outline a possible application in which well-composed polyhedral complexes can be useful.Junta de Andalucía FQM-369Ministerio de Economía y Competitividad MTM2012-32706Ministerio de Economía y Competitividad MTM2015-67072-

Spatiotemporal Barcodes for Image Sequence Analysis

Taking as input a time-varying sequence of two-dimensional (2D) binary images, we develop an algorithm for computing a spatiotemporal 0–barcode encoding lifetime of connected components on the image sequence over time. This information may not coincide with the one provided by the 0–barcode encoding the 0–persistent homology, since the latter does not respect the principle that it is not possible to move backwards in time. A cell complex K is computed from the given sequence, being the cells of K classified as spatial or temporal depending on whether they connect two consecutive frames or not. A spatiotemporal path is defined as a sequence of edges of K forming a path such that two edges of the path cannot connect the same two consecutive frames. In our algorithm, for each vertex v ∈ K, a spatiotemporal path from v to the “oldest” spatiotemporally-connected vertex is computed and the corresponding spatiotemporal 0–bar is added to the spatiotemporal 0–barcode.Junta de Andalucía FQM-369Ministerio de Economía y Competitividad MTM2012-3270

Un retablo del maestro Antonio José Proenza en la iglesia parroquial de San Miguel Arcángel de Solana de Cabañas (Cáceres)

Descripción y análisis del retablo dedicado a Santa Lucía en la iglesia parroquial de San Miguel Arcángel de Solana de Cabañas (Cáceres), obra del escultor portugués Antonio José Proenza, a finales del siglo XVIII, a través de la documentación que se conserva en su archivo.Description and analysis of the altarpiece dedicated to Saint Lucia in the parish church of Saint Michael the Archangel in Solana de Cabañas (Cáceres), work of the Portuguese sculptor Antonio José Proenza, at the end of the XVIIIth century, through the documentation kept in its archive.peerReviewe